China Shantui wheel loader mini 3 ton compact loader SL30WN on

  • class - Stack Overflow

    Nov 10, 2012 · Stack Overflow Public questions & answers; There are two main places to extend a class loader to change the way classes are loaded: findClass(String name) - You override this method when you want to find a class with the usual parent first delegation. loadClass(String name, boolean resolve) - Override this method when you want to change the

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  • DNA entry, exit and second DNA capture by cohesin

    Jan 01, 2018 · Scc2, with its stable binding partner Scc4, promotes cohesin loading whereas Pds5, with its substoichiometric binding partner Wapl, dissociates cohesin from chromatin [31 – 33]. If the molecular function of cohesin involves topological embrace, the ring must be opened for both DNA entrapment and release.

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  • Biochemical reconstitution of topological DNA binding by

    Dec 01, 2013 · The cohesin loader binds to DNA. We purified the fission yeast Mis4 Scc2 /Ssl3 Scc4 cohesin loader complex after overexpression of its two subunits in fission yeast (Fig. 1a and Extended Data Fig. 1a).We used a similar strategy to purify the large Mis4 Scc2 subunit by itself. Based on its hydrodynamic properties, Mis4 Scc2 /Ssl3 Scc4 is a moderately elongated, heterodimeric protein …

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  • New insights into cohesin loading | SpringerLink

    Jun 19, 2017 · Cohesin is a conserved, ring-shaped protein complex that encircles sister chromatids and ensures correct chromosome segregation during mitosis and meiosis. It also plays a crucial role in the regulation of gene expression, DNA condensation, and DNA repair through both non-homologous end joining and homologous recombination. Cohesins are spatiotemporally regulated by the Scc2–Scc4 …

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  • Cohesin loading and sliding | Journal of Cell Science

    The Smc5–Smc6 complex has important roles in DNA repair, probably in part through its association with specific additional subunits that participate in DNA repair. Its binding to chromosomes along chromosome arms, but not at sites of DNA damage, depends on Scc2–Scc4 (Betts Lindroos et al., 2006). The association pattern of the Smc5–Smc6

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  • Intermediate step of cohesin's ATPase cycle allows cohesin

    Sep 25, 2018 · (A) Effect of ADP/AlFx on loader binding to cohesin using DNA-bead assay. WT cohesin and an equimolar amount of loader was assayed for binding to DNA beads in the presence or ATP or ADP/AlFx. Silver-stained gel to assess cohesin (Psm3, Psm1, Rad21/Psc3) and loader (Mis4, Ssl3) bound to …

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  • Biochemical reconstitution of topological DNA binding by

    Dec 01, 2013 · Biochemical reconstitution of topological DNA binding by the cohesin ring DNA using purified fission yeast cohesin and its loader complex, Mis4(Scc2)-Ssl3(Scc4) (Schizosaccharomyces pombe …

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  • Condensin complexes: understanding loop extrusion one

    DNA binds to Ycs4 in the condensin bridged state. The hinge could additionally contribute to DNA binding. DNA binding promotes closure of the ATPase heads, resulting in DNA trapped within both the K and the S compartment. Proximity of ATPase heads promote ATP binding, release of Ycs4 and binding of Ycg1 to the Smc2 ATPase heads.

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  • DNA Binding by the Mis4 Scc2 Loader Promotes …

    Mis4Scc2 binds to DNA via two distinct sites. The DNA binding activity of Mis4Scc2 strongly correlates with its ability to stimu-late topological cohesin loading onto DNA. Furthermore, the Mis4Scc2 loaderwasfoundtoformatertiarycomplexwithcohesin on DNA, where its DNA binding activity facilitates the initial DNA binding of cohesin.

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  • A Structure-Based Mechanism for DNA Entry into the Cohesin

    Sep 17, 2020 · A protein ring, partly formed by Mis4 in the gripping state, is unexpected and distinct from the well-established cohesin ring. Our structure explains why ATP binding and not hydrolysis is sufficient for topological DNA binding (Çamdere et al., 2018; Minamino et al., 2018). Thus, two distinct topological interactions are formed between protein

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  • Topological entrapment of DNA by cohesin | Crick

    The resulting atomic model covered the two Psm head domains and adjacent coiled coil, bound to the kleisin N- and C-termini, as well as the Mis4 loader and 32bp of DNA. The structure gave unprecedented insight into mechanism. Mis4 was clamping the DNA onto the ATPase head domains, making widespread contacts with both Psm1 and Psm3.

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  • DNA Binding by the Mis4 Scc2 Loader Promotes Topological

    Two distinct sites within the Mis4 Scc2 subunit are found to cooperatively bind DNA. Mis4 Scc2 initially forms a tertiary complex with cohesin on DNA and promotes subsequent topological DNA entrapment by cohesin through its DNA binding activity, a process that requires an additional DNA binding surface provided by Psm3 Smc3, the ATPase domain of cohesin. Furthermore, we show that mutations in the …

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  • Conserved features of cohesin binding along fission yeast

    May 19, 2009 · Little is known about the Mis4/Ssl3 cohesin loader (orthologs of budding yeast Scc2/Scc4), a protein complex that is required for cohesin's association with chromosomes [3, 22, 23]. How the cohesin loader recognizes its binding sites on chromosomes, and how it promotes cohesin loading at these sites, are poorly understood.

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  • python - PyTorch ImageFolder to DataLoader Changes Image

    I use DataLoader and I wanted to check to make sure the input shape remained intact. train_data_loader = data.DataLoader (dataset, batch_size=1, shuffle=True) inputs, classes = next (iter (train_data_loader)) inputs.shape. which reveals the shape is [10, 3, 360, 1080]. It seems that there is a lot of padding being added here given the last two

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  • DNA Binding by the Mis4Scc2 Loader Promotes …

    Cohesin, a critical mediator of genome organization including sister chromatid cohesion, is a ring-shaped multi-subunit ATPase that topologically embraces DNA. Its loading and fun

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  • Cell Reports | Vol 33, Issue 6, 10 November 2020

    Nov 10, 2020 · Pairwise Synchrony and Correlations Depend on the Structure of the Population Code in Visual Cortex. Veronika Koren, Ariana R. Andrei, Ming Hu, Valentin Dragoi, Klaus Obermayer. Article 108367. Download PDF.

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  • mis4 - Sister chromatid cohesion protein mis4

    Mar 21, 2012 · single-stranded DNA binding Source: PomBase "DNA Binding by the Mis4<sup>Scc2</sup> Loader Promotes Topological DNA Entrapment by the Cohesin Ring." Kurokawa Y., Murayama Y. Cell Rep 33:108357-108357(2020) [ PubMed ] [ Europe PMC ] [ Abstract ]

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  • Establishment of DNA-DNA Interactions by the Cohesin Ring

    Jan 18, 2018 · The cohesin ring is loaded onto chromatin well before DNA replication with the aid of a cohesin loader complex, a heterodimer of Scc2 and Scc4 (Mis4 and Ssl3) (Bernard et al., 2006, Furuya et al., 1998). The cohesin loader has been suggested to stabilize a cohesin conformation that exposes two DNA sensory lysines on the Smc3 ATPase head.

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  • Biochemical reconstitution of topological DNA binding by

    Dec 01, 2013 · Mis4 Scc2 /Ssl3 Scc4 is a DNA binding protein. a, Purified Mis4 Scc2 /Ssl3 Scc4 complex (M4S3) and its Mis4 Scc2 subunit (M4) were analyzed by SDS-PAGE and Coomassie blue staining (CBB) or Western blotting using antibodies directed against Mis4 or its C-terminal HA epitope. b, glycerol gradient centrifugation of the Mis4 Scc2 /Ssl3 Scc4 complex, followed by SDS-PAGE and silver staining.

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  • Biochemical reconstitution of topological DNA binding by

    Cohesion between sister chromatids, mediated by the chromosomal cohesin complex, is a prerequisite for faithful chromosome segregation in mitosis. Cohesin also has vital roles in DNA repair and transcriptional regulation. The ring-shaped cohesin complex is thought to encircle sister DNA strands, but its molecular mechanism of action is poorly understood and the biochemical reconstitution of

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